RN 1734
2,4-dichloro-N-isopropyl-N-[2-(isopropylamino)ethyl]benzenesulfonamide
CAS: 946387-07-1
Molecular Formula: C14H22Cl2N2O2S
RN 1734 - Names and Identifiers
RN 1734 - Physico-chemical Properties
| Molecular Formula | C14H22Cl2N2O2S |
| Molar Mass | 353.31 |
| Solubility | DMSO: >20mg/mL |
| Appearance | Yellow solid |
| Color | white to off-white |
| Storage Condition | 2-8°C |
| In vitro study | RN-1734 (27 hours; 10μM) reverses the increase in the apoptotic rate of oligodendrocytes induced by CM (LPS-activated microglia group) apoptosis. RN-1734 (27 hours; 10μM) alleviates CM-induced decreases in CNP. Apoptosis Analysis Cell Line: Microglial cells Concentration: 27 hours Incubation Time: 10μM Result: Significantly decreased the percentage of cleaved-caspase 3 positive cells. Western Blot Analysis Cell Line: Microglial cells Concentration: 27 hours Incubation Time: 10 μM Result: Alleviated CM (with LPS only)-induced decreases in CNP. |
| In vivo study | RN-1734 (0.5 μl; microinjector pump; daily for 5 weeks) significantly reverses the decrease in CNP protein and improves myelination in CPZ-induced demyelination mouse. Animal Model: CPZ-induced demyelination mouse model (C57BL/6 male mice) Dosage: 0.5 μl (10 μM in 5% DMSO and 0.9% NaCl) Administration: Microinjector pump for 5 weeks Result: Significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse. |
RN 1734 - Risk and Safety
| WGK Germany | nwg |
RN 1734 - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 2.833 ml | 14.164 ml | 28.329 ml |
| 5 mM | 0.567 ml | 2.833 ml | 5.666 ml |
| 10 mM | 0.283 ml | 1.416 ml | 2.833 ml |
| 5 mM | 0.057 ml | 0.283 ml | 0.567 ml |
Last Update：2024-01-02 23:10:35
RN 1734 - Reference Information
| biological activity | RN-1734 (TRPV4 Antagonist I) is a selective transient receptor potential vanilloid 4 (TRPV4) antagonist, and the IC50 values for hTRPV4, mTRPV4 and rTRPV4 are 2.3 μM, 5.9 μM and 3.2 μM respectively. RN-1734, without changing the number of olig2-positive cells, attenuated demyelination and inhibited glial activation as well as inhibition of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) production. |
| target | TargetValue TNF-α () IL-1β () hTRPV4 (Cell-free assay) 2.3 μM rTRPV4 (Cell-free assay) 3.2 μM TRPV4 (Cell-free assay) 5.9 μM |
| Target | Value |
| TNF-α () | |
| IL-1β () | |
| hTRPV4 (Cell-free assay) | 2.3 μM |
| rTRPV4 (Cell-free assay) | 3.2 μM |
| mTRPV4 (Cell-free assay) | 5.9 μM |
| in vitro study | RN-1734 (27 hours; 10 μM) reverses the increase in the apoptotic rate of oligodendrocytes induced by CM (LPS-activated microglia group) apoptosis. RN-1734 (27 hours; 10 μM) alleviates CM-induced decreases in CNP. Apoptosis Analysis Cell Line: Microglial cells Concentration: 27 hours Incubation Time: 10 μM Result: Significantly decreased the percentage of cleaved-caspase 3 positive cells. Western Blot Analysis Cell Line: Microglial cells Concentration: 27 hours Incubation Time: 10 μM Result: alleviated CM (with LPS only)-induced decreases in CNP. |
| Cell Line: | Microglial cells Microglial cells |
| Concentration: | 27 hours 27 hours |
| Incubation Time: | 10μM 10 μM |
| Result: | Significantly decreased the percentage of cleaved-caspase 3 positive cells. Alleviated CM (with LPS only)-induced decreases in CNP. Significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse. |
| in vivo research | RN-1734 (0.5 μl; microinjector pump; Daily for 5 weeks) significantly reverses the decrease in CNP protein and improves myelination in CPZ-induced demyelination mouse. Animal Model: CPZ-induced demyelination mouse model (C57BL/6 male mice) Dosage: 0.5 μl (10 μM in 5% DMSO and 0.9% NaCl) Administration: Microinjector pump for 5 weeks Result: significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse. |
| Animal Model: | CPZ-induced demyelination mouse model (C57BL/6 male mice) |
| Dosage: | 0.5 μl (10 μM in 5% DMSO and 0.9% NaCl) |
| Administration: | Microinjector pump for 5 weeks |
Last Update：2024-04-10 22:29:15
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Spot supply
Product Name: 2,4-dichloro-N-isopropyl-N-[2-(isopropylamino)ethyl]benzenesulfonamide Visit Supplier Webpage Request for quotationCAS: 946387-07-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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